Tape Strips Identified as Non-Invasive Approach to Assess Skin Biomarkers in Children With Atopic Dermatitis
May also help predict therapeutic responses
Applying strips of adhesive tape to skin lesions and non-lesional skin is a non-invasive way to obtain biomarkers to track the severity of early-onset pediatric eczema or atopic dermatitis (AD) in infants and young children, researchers report for the first time. The study, titled “Use of Tape Strips to Detect Immune and Barrier Abnormalities In Early Onset Pediatric Atopic Dermatitis” and led by researchers at the Icahn School of Medicine at Mount Sinai and Northwestern University’s Feinberg School of Medicine, was published online today in Jama Dermatology.
According to the lead author of the paper, Emma Guttman-Yassky, MD, PhD, Sol and Clara Kest Professor and Vice Chair of Dermatology, and Professor of Immunology, at the Icahn School of Medicine, this is the first study to provide a broad characterization of immune and barrier abnormalities in the skin of children with early-onset eczema, those aged five and under who have had eczema for less than six months.
“Up until now, skin tissue biopsies have been considered the gold standard for evaluating dysregulation in lesional and non-lesional AD/eczema skin,” said Dr. Guttman-Yassky. “Therefore, it has been crucial to understand whether profiling of the tiny amounts of RNA obtained from the outer layers of the skin using tape strips can accurately yield key biomarkers for AD.”
Dr. Guttman-Yassky and her team of researchers at the Icahn School of Medicine at Mount Sinai discovered that tape strips applied several times to the same skin areas provide a perfect classifier to discriminate between early-onset AD skin and normal pediatric skin, including biomarkers pathogenically linked to AD. “This work can be highly informative for longitudinal studies in infants and children and in clinical trials for both children and adults,” said Dr. Guttman-Yassky, whose past revolutionary research on AD/eczema focused on the mechanism underlying the disease and promoted development of targeted therapeutics for eczema, and who has identified biomarkers to track improvement with current treatments for the disease.
Atopic dermatitis is an inflammatory, extremely itchy skin disorder that affects 10 to 20 percent of children in the United States and more than 31 million adults.
Amy S. Paller, MD, Walter J. Hamlin Professor and Chair of Dermatology at Northwestern and a senior author, said, “While skin biopsies are simple to perform in adults as a way to obtain skin to assess for biomarkers that allow prediction of course and responses to targeted medications, they are nearly impossible to obtain in children, especially small children. We have now demonstrated that tape strips can be used to obtain skin for quantifying expression of numerous genes that are important markers of both immune and barrier function in AD in young children.”
The researchers investigated a total of 51 children younger than five, some with moderate to severe eczema and others without eczema, from an outpatient dermatology clinic at a children’s hospital. Sixteen tape strips were collected from each of the skin of 21 lesional and non-lesional children who had AD and were less than six months from disease initiation, and from the skin of 30 normal children who did not have AD, between January 2016 and April 2018. Gene and protein expression were then evaluated using real-time polymerase chain reaction and immunohistochemistory.
Said Dr. Guttman-Yassky, “Being able to noninvasively collect skin in children for this study paves the way for future studies to better understand a wide variety of skin disorders as well as to start identifying markers to predict course, comorbidities, and treatment response in prospective studies.”
Additionally, the same tape strips can be used for collection and analysis of the skin microbiome (bacterial and viral inhabitants of the skin’s surface), allowing investigations that compare changes in the skin microbial population and gene/protein changes.
Mark Lebwohl, MD, Waldman Professor of Dermatology and Chair of the Kimberly and Eric J. Waldman Department of Dermatology at the Icahn School of Medicine at Mount Sinai, said, “This important study emphasizes the great need of better understanding immune and barrier alterations in pediatric skin. Dr. Guttman-Yassky’s revolutionary research is continuing to open the door to new therapeutic discoveries by helping to improve the quality of lives of both adults and children with eczema and beyond.
The study was supported by grant NIAMS P30 ARO57216 from the Northwestern University Skin Disease Research Center and grant UL1TR001422 from the Northwestern Clinical Translational Sciences Institute. Research was supported in part by a grant from Regeneron and Sanofi.
About the Mount Sinai Health System
The Mount Sinai Health System is New York City's largest integrated delivery system, encompassing eight hospitals, a leading medical school, and a vast network of ambulatory practices throughout the greater New York region. Mount Sinai's vision is to produce the safest care, the highest quality, the highest satisfaction, the best access and the best value of any health system in the nation. The Health System includes approximately 7,480 primary and specialty care physicians; 11 joint-venture ambulatory surgery centers; more than 410 ambulatory practices throughout the five boroughs of New York City, Westchester, Long Island, and Florida; and 31 affiliated community health centers. The Icahn School of Medicine is one of three medical schools that have earned distinction by multiple indicators: ranked in the top 20 by U.S. News & World Report's "Best Medical Schools", aligned with a U.S. News & World Report's "Honor Roll" Hospital, No. 12 in the nation for National Institutes of Health funding, and among the top 10 most innovative research institutions as ranked by the journal Nature in its Nature Innovation Index. This reflects a special level of excellence in education, clinical practice, and research. The Mount Sinai Hospital is ranked No. 14 on U.S. News & World Report's "Honor Roll" of top U.S. hospitals; it is one of the nation's top 20 hospitals in Cardiology/Heart Surgery, Diabetes/Endocrinology, Gastroenterology/GI Surgery, Geriatrics, Gynecology, Nephrology, Neurology/Neurosurgery, and Orthopedics in the 2019-2020 "Best Hospitals" issue. Mount Sinai's Kravis Children's Hospital also is ranked nationally in five out of ten pediatric specialties by U.S. News & World Report. The New York Eye and Ear Infirmary of Mount Sinai is ranked 12th nationally for Ophthalmology and Mount Sinai South Nassau is ranked 35th nationally for Urology. Mount Sinai Beth Israel, Mount Sinai St. Luke's, Mount Sinai West, and Mount Sinai South Nassau are ranked regionally.