Ear drainage culture
Culture - ear drainage
An ear drainage culture is a lab test. This test checks for germs that can cause infection. The sample taken for this test can contain fluid, pus, wax, or blood from the ear.

The ear consists of external, middle, and inner structures. The eardrum and the three tiny bones conduct sound from the eardrum to the cochlea.

The external structures of the ear may aid in diagnosing some conditions by the presence or absence of normal landmarks and abnormal features including earlobe creases, preauricular pits, and preauricular tags.

An ear drainage culture is collected by placing a cotton swab gently in the ear canal. The sample is sent to the laboratory for testing to isolate and identify the type of organism causing the ear infection.
How the Test is Performed
A sample of ear drainage is needed. Your health care provider will use a cotton swab to collect the sample from inside the outer ear canal. In some cases, a sample is collected from the middle ear during ear surgery.
The sample is sent to a lab and placed on a special dish (culture media).
The lab team checks the dish every day to see if bacteria, fungi, or viruses have grown. More tests may be done to look for specific germs and determine the best treatment.
How to Prepare for the Test
You do not need to prepare for this test.
How the Test will Feel
Using a cotton swab to take a sample of drainage from the outer ear is not painful. However, ear pain may be present if the ear is infected.
Ear surgery is done using general anesthesia. You will be asleep and feel no pain.
Why the Test is Performed
The test may be done if you or your child has:
- An ear infection that is not getting better with treatment
- An infection of the outer ear (otitis externa)
- An ear infection with a ruptured eardrum and draining fluid
It may also be done as a routine part of myringotomy.
Note: Ear infections are diagnosed based on symptoms rather than using a culture.
Normal Results
The test is normal if there is no growth on the culture.
Normal value ranges may vary slightly among different laboratories. Talk to your provider about the meaning of your specific test results.
What Abnormal Results Mean
Abnormal results may be a sign of an infection. The infection can be caused by bacteria, viruses, or fungi.
The test results may show which organism is causing the infection. It will help your provider decide on the right treatment.
Risks
No risks are involved with swabbing the ear canal. Ear surgery may involve some risks.
References
Pelton SI. Otitis externa, otitis media, and mastoiditis. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Philadelphia, PA: Elsevier; 2020:chap 61.
Player B. Otitis media. In: Kliegman RM, St. Geme JW, Blum NJ, et al, eds. Nelson Textbook of Pediatrics. 22nd ed. Philadelphia, PA: Elsevier; 2025:chap 680.
Schilder AGM, Rosenfeld RM, Venekamp RP. Acute otitis media and otitis media with effusion. In: Flint PW, Francis HW, Haughey BH, et al, eds. Cummings Otolaryngology: Head and Neck Surgery. 7th ed. Philadelphia, PA: Elsevier; 2021:chap 199.
Version Info
Last reviewed on: 7/31/2024
Reviewed by: Charles I. Schwartz, MD, FAAP, Clinical Assistant Professor of Pediatrics, Perelman School of Medicine at the University of Pennsylvania, General Pediatrician at PennCare for Kids, Phoenixville, PA. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.
