"Scientists Develop Universal FACS-based Approach to Heterogenous Cell Sorting, Propelling Organoid Research" - Hidaya Aliouche
Researchers at the Icahn School of Medicine at Mount Sinai have developed a series of novel fluorescence‐assisted cell sorting protocols that enable isolation of neural progenitors, astrocytes, and neurons from virus‐infected cerebral organoids, for downstream multi-omics analysis. “FACS over microscopy‐based cell identification not only reduces output quantification errors, but also expands choices for downstream analyses of the identified cell,” said Sylvie Janssens, PhD, postdoctoral fellow of developmental and regenerative biology at the Icahn School of Medicine at Mount Sinai. The team developed three protocols for the FACS based identification and isolation of specific neuronal cell populations from virus-infected cerebral organoids.
— Sylvie Janssens, PhD, Postdoctoral Fellow, Developmental and Regenerative Biology, Icahn School of Mount Sinai