Subspecialty	Clinical and Laboratory Immunology - Pediatrics
Gender	Male
E-mail	natasha.ilievski@mssm.edu
	hugh.sampson@mssm.edu
Education and Training	MD, S.U.N.Y., Buffalo
	Residency, Pediatrics, Children's Memorial Hospital
	Fellowship, Allergy & Immunology, Duke University Hospital
Awards	2004 Brett Ratner Award Outstanding Research in Pediatric Allergy/Immunology American Academy of Pediatrics
	2003 - present Outstanding Research in Food Hypersensitivity Disorders Institute of Medicine; National Academy of Science
	2002 International Award in Nutrition Outstanding research in the area of milk allergy
	2001 - 2008 New York Top Doctors NY Magazine's top clinical doctors in New York
	1986 - 1991 Allergic Disease Academic Award NIAID competitive award for research in food allergy

Dr. Sampson is a Professor of Pediatrics and Immunobiology at the Mount Sinai School of Medicine. He is Chief of the Division of Allergy & Immunology in the Department of Pediatrics, Director of the Jaffe Food Allergy Institute, and Dean of Translational Biomedical Science at The Mount Sinai Medical Center. Dr. Sampson's research interests have focused on food allergic disorders, including work on the immuno-pathogenic role of food hypersensitivity in atopic dermatitis, the pathogenesis of food-induced anaphylaxis, characterization of food-induced gastrointestinal hypersensitivities, characterization of food allergens, and novel immunotherapeutic strategies (recombinant engineered protein, plasmid DNA, peptide, etc.) for treating food allergies. His research has been funded by a number of grants from the National Institutes of Health (R01 AI 24439, R01 AI 43668 and P01 AI 44236) and private foundations (Clarissa Sosin Foundation and Food Allergy Initiative), and is the PI on the NIH-sponsored Consortium of Food Allergy Research. Dr. Sampson supervises one clinic/week in pediatric allergy.

In the News

Dr. Sampson talks about food allergies in The Daily News feature The Daily Check Up. View the PDF.

Education and Training	MD, S.U.N.Y., Buffalo
	Residency, Pediatrics, Children's Memorial Hospital
	Fellowship, Allergy & Immunology, Duke University Hospital

Subspecialty	Clinical and Laboratory Immunology - Pediatrics

Specific Clinical/Research Interest: Immunopathogenic mechanisms of food allergic disorders and asthma; immunomodulatory therapies

Current Students: PhD: Kamal Srivastava

Postdoctoral Fellows: Katherine Bloom, Jing S. Lin

Research Personnel: Alexander Grishin, PhD; Luda Bardina, MS; Galina Grishin, MS; Russell Castro, BS; Yesim Kucuk, MD

Summary of Research Studies:
Our laboratory is evaluating immunopathologic mechanisms of food allergic disorders. Specifically we are identifying allergenic proteins at a molecular and structural level, and investigating the interaction between IgE antibodies and allergenic proteins and the immune response at a cellular and molecular level. Studies utilize patient specimens and murine models in an attempt to elucidate underlying mechanisms. Allergenic proteins in egg, milk, peanut, several tree nuts, shrimp and some fish have been fully characterized and full-length cDNAs isolated and cloned. A number of therapeutic strategies are under investigation utilizing murine models of anaphylaxis and asthma including the use of recombinant proteins, DNA vaccines, and CpG-conjugated proteins. Several early stage, human clincial trials are in progress to treat food allergic disorders including oral and sublingual immunotherapy, herbal therapies, and engineered recombinant protein vaccines.

In addition, our laboratory is serving as the mechanistic center for the NIAID Inner City Asthma Consortium and the Consortiium for Food Allergy Research. The asthma consortium is investigating the role of allergic sensitization in inner city children and its potential role in the increased morbidity and mortality found in this population. The overall goal is to determine whether the nature and quantity of environmental allergens within the inner city, especially cockroach, are unique in their ability to determine and drive the intensity of allergic inflammation in sensitized children residing in the inner city and thus the severity of their asthma.

The food allergy consortium is investigating the immunologic mechanisms associated with the devleopment of peanut allergy and the development of tolerance ["outgrowing"] to egg and milk allergy, the immunologic consequences of oral immunotherapy for egg allergy, and the immunologic consequences of sublingual immunotherapy for peanut allergy. The lab employs a variety of techniques to identify and purify proteins including SDS-PAGE, immunoblotting and HPLC. Recombinant proteins are generated from cDNA isolated from appropriate cDNA libraries. A variety of techniques are utilized to study both humoral and cellular responses of patient groups and controls. Characterization of cellular responses includes intracytoplasmic staining, mRNA generation, and characterization of cytokines secreted into cell supernatants. Similar studies are conducted in the murine models.

Jaffe Food Allergy Institute

Shreffler WG, Lencer DA, Bardina L, Sampson HA. IgE and IgG4 epitope mapping by microarray immunoassay reveals the diversity of immune response to the peanut allergen, Ara h 2. J Allergy Clin Immunol 2005; 116: 893-899.

Jarvinen KM, Beyer K, Vila L, Bardina L, Mishoe M, Cooke SH, Sampson HA. Specificity of IgE antibodies to linear epitopes of hen's egg ovomucoid as a marker for persistence of egg allergy. Allergy 2007; 62: 758-765.

Beyer K, Grishina G, Bardina L, Sampson HA. Identification of two new sesame seed allergens -Ses i 6 and Ses i 7. J Allergy Clin Immunol 2007; 119: 1554-1556.

Chehade M, Sampson HA, Morotti RA, Magid MS. Esophageal subepithelial fibrosis in children with eosinophilic esophagitis. J Pediatr Gastroenterol Nutr 2007 Sep; 45(3): 319-328.

Han N, Jarvinen KM, Cocco RR, Busse PJ, Sampson HA, Beyer K. Identification of amino acids critical for IgE-binding to sequential epitopes of bovine kappa-casein and the similarity of these epitopes to the corresponding human kappa-casein sequence. Allergy 2008 Feb; 63(2): 198-204.

Jarvinen KM, Han N, Cocco R, Busse PJ, Sampson HA, Beyer K. Identification of Amino Acids Critical for IgE-binding to Bovine-Casein and Potential homology between of IgE-binding Epitopes of Cow's Milk Caseins and Caseins of Man. Allergy 2008; 63(2): 198-204.

Nowak-Wegrzyn A, Bloom KA, Sicherer SH, Shreffler WG, Noone S, Wanich N, Sampson HA. Tolerance to extensively heated milk in children with cow's milk allergy. J Allergy Clin Immunol 2008 Aug; 122(2): 342-347.

Cerecedo I, Zamora J, Shreffler WG, Lin J, Bardina L, Dieguez MC, Wang J, Muriel A, de la Hoz B, Sampson HA. Mapping of the IgE and IgG4 sequential epitopes of milk allergens with a peptide microarray-based immunoassay. J Allergy Clin Immunol 2008 Sep; 122(3): 589-594.

Lemon-Mule H, Sampson HA, Sicherer SH, Shreffler WG, Noone S, Nowak-Wegrzyn A. Immunologic changes in children with egg allergy ingesting extensively heated egg. J Allergy Clin Immunol 2008 Nov; 122(5): 977-983.

Shreffler WG, Wanich N, Maloney M, Nowak-Wegrzyn A, Sampson HA. The Association of Allergen-Specific Regulatory T Cells with the Onset of Clinical Tolerance to Milk Protein. J Allergy Clin Immunol 2009 Jan; 123(1): 43-52.