Gender	Female
E-mail	kirsten.edepli@mssm.edu
Education and Training	Ph.D., Harvard University
	M.M.Sc., Harvard Medical School
	B.A., Mount Holyoke College
	Fellowship, Massachusetts Institute of Technology
Awards	2008 - 2011 Research Scholar Award American Gastroenterological Association
	2008 - 2011 Basil O'Connor Starter Scholar Award March of Dimes
	2008 - 2010 Young Investigator Award Breast Cancer Alliance

Department of Developmental & Regenerative Biology

Zebrafish Models of Liver Disease Laboratory

Mount Sinai Alcoholic Liver Disease Research Center

Education and Training	Ph.D., Harvard University
	M.M.Sc., Harvard Medical School
	B.A., Mount Holyoke College
	Fellowship, Massachusetts Institute of Technology

Dr. Sadler-Edepli is the Director of the Zebrafish Models of Liver Disease Laboratory and the Model Co-Core Director of the Mount Sinai Alcoholic Liver Disease Research Center. 

Specific Clinical/Research Interest: Using zebrafish to understand liver development, regeneration and disease
Current Students: Raksha Mudbhary, Dru Imrie

Postdoctoral Fellows: Ayca Cinaroglu, Chuan Gao, Jaime Chu

Research Personnel: Liz Loughlin, Chris Monson

Current Research Focus
Using zebrafish to understand liver development, regeneration and disease

Zebrafish are an excellent model for studying embryonic development and we are using the power of zebrafish genetics to define genes required for liver growth as well as to identify new models of liver diseases. Fatty liver disease is emerging as an important liver pathology and is typically associated with obesity and type II diabetes and together these comprise Metabolic Syndrome, which affects nearly 5 percent of the American population. We have found a zebrafish mutant that develops fatty liver disease in the embryo, and have named it foie gras (fgr). The foie gras gene is well conserved in animals, but has no assigned sequence or motif that suggest its function. The primary focus of the Sadler Edepli lab is to understand the cellular function of foie gras, and to use the fgr mutant embryo as a model for studying fatty liver disease.

The second focus of our lab is to determine the genetic basis for liver growth in the embryo and during regeneration in adults, and is aimed at testing the hypothesis that a similar program may be responsible for growth control in both circumstances. We have carried out a screen to identify zebrafish mutant embryos which fail to undergo liver growth and are testing the genes responsible for the embryonic phenotype for their role in liver regeneration following partial hepatectomy.

Current Research Studies
1. Breast Cancer Alliance; Young Investigator Award   01/2008-12/2010
PI: KC Sadler
Identifying UHRF1 target genes in breast cancer and embryogenesis
Goal: UHRF1 is a proposed oncogene in breast cancer and plays a role in embryogenesis and the main goal of this project is to identify downstream target genes that are relevant to breast cancer
 
2. 5-FY07-670 March of Dimes; Basil O'Connor Starter Scholar Award 02/2008-01/2011
PI: KC Sadler
Uncovering mechanisms of uhrf1 action in liver development
Goal: To identify downstream target genes of UHRF1 that are relevant to liver development in zebrafish.
 
3. American Gastroenterological Association; Research Scholar Award  07/2008-06/2011
PI: KC Sadler
Using zebrafish to uncover the role of the unfolded protein response in steatosis
Goal: To delineate the contribution of protein glycosylation defects and activation of the unfolded protein response to the development of fatty liver disease in zebrafish
 
4. 1 P20 AA017067-01 NIAA/NIH 07/2008-06/2013
PI: Scott L. Friedman; KC Sadler, subcontractor
Oxidant Stress and Fibrosis in Alcoholic Liver Injury
Goal: To develop an infrastructure for the establishment of an exploratory Alcohol Research Center that focuses on understanding the mechanisms of alcoholic liver injury and fibrosis. My specific role in this project is to develop a zebrafish model of alcoholic liver disease, and to administer a zebrafish core facility.
 
5. Experimental Therapeutics Institute, Mount Sinai School of Medicine 01/2009-12/2009
Co-PI: KC Sadler and Dr. R. Cagan
A Novel Screen for Therapeutics to Treat Fatty Liver Disease

Passeri M, Cinaroglu A, Gao C, Sadler KC. Hepatic Steatosis in Response to Acute Alcohol Exposure in Zebrafish Depends Upon SREBP Activation. Hepatology 2008; 49.

Toyoshima Y, Monson C, Duan C, Wu Y, Gao C, Yakar S, Sadler KC, LeRoith D. The role of insulin receptor signaling in zebrafish embryogenesis. Endocrinology 2008; 149: 5996-6005.

Sakagachi T, Sadler KC, Crosnier C, Stainier D. Endothelial signals modulate hepatocyte apico-basal polarization in zebrafish. Current Biology 2007; 18(20): 1565-1571.

Sadler KC, Krahn KN, Gaur NA, Ukomadu C. Liver growth in the embryo and during liver regeneration in zebrafish requires the cell cycle regulator, uhrf1. Proc Natl Acad Sci U S A 2007; 104: 1570-1575.

Sadler-Edepli KC, Amsterdam A, Soroka C, Boyer J, Hopkins N. A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease. Development 2005 Aug; 132(15): 3561-72.

Amsterdam A, Sadler-Edepli KC, Lai K, Farrington S, Bronson RT, Lees JA, Hopkins N. Many ribosomal protein genes are cancer genes in zebrafish. PLoS Biol 2004 May; 2(5): E139.