Specialty	Anatomic Pathology
Gender	Male
E-mail	david.zhang@mssm.edu
Education and Training	MD, Norman Bethune Univ of Medical Sciences
	Ph.D., NYU School of Medicine
	MPH, Mount Sinai School of Medicine
	Residency, Pathology, Mount Sinai Hospital
	Residency, Occupational Medicine, Mount Sinai Hospital
	Fellowship, Cytopathology, Mount Sinai Hospital
	Fellowship, Clinical Microbio, Mount Sinai Hospital

Education and Training	MD, Norman Bethune Univ of Medical Sciences
	Ph.D., NYU School of Medicine
	MPH, Mount Sinai School of Medicine
	Residency, Pathology, Mount Sinai Hospital
	Residency, Occupational Medicine, Mount Sinai Hospital
	Fellowship, Cytopathology, Mount Sinai Hospital
	Fellowship, Clinical Microbio, Mount Sinai Hospital

Specialty	Anatomic Pathology

Certifications:
Anatomic and Clinical Pathology, Cytopathology, Molecular Genetic Pathology, Preventive Medicine (Occupational Medicine)

Specific Clinical/Research Interest:
Molecular cancer biology with focus on signal transduction pathways and technology development for cancer early diagnosis.

Current Students: Hongxiao Wang, Ling Gao

Postdoctoral Fellows: Kai Zhang, Xiaoliang Liu, Norma Steiner

Research Personnel: Fei Ye PhD, Clinical Assistant Professor; Josephine Wu DDS, Assistant professor

Summary of Research Studies:
We developed a signal transduction pathway-focused proteomic method, termed PATHWAY ARRAY, which can be used to globally screen the signaling proteins and their activation. With the assistance of computation, we are able to build a signaling network that controls cell proliferation, apoptosis, angiogenesis, necrosis, etc. This method has been used to identify novel candidate proteins as cancer biomarkers and/or targets for cancer treatment. This method was also used to understand molecular mechanism of the compounds isolated from medicinal plants, such as Scutellaria baicalensis. These plants and the ingredients have strong anticancer activities against various cancers, including prostate, breast, colon, and lung cancers. The plants or their ingredients may be used as novel chemopreventive and chemotherapeutic agents for cancer prevention and treatment. We also invented several DNA amplification technologies, including the rolling circle amplification (RCA), isothermal ramification amplification assay (RAM) and hybridization signal amplification (HSAM). These technologies have been granted by the US Patent Office and licensed to Hamilton Thorne Biosciences, a biotechnology company based in Boston. RAM, unlike conventional polymerase chain reaction (PCR), can amplify DNA, RNA and protein without the use of thermocycling. It is extremely sensitive and can detect as few as 10 molecules. HSAM is another signal amplification technology based on the principles of nucleic acid hybridization and specific ligand interaction. This technology is simple and sensitive to identify DNA/RNA targets and proteins and can be apply to in situ amplification, DNA arrays, and proteomics.

Yang C, Lu P, Lee FY, Chadburn A, Barrientos JC, Leonard JP, Ye F, Zhang D, Knowles DM, Wang YL. Tyrosine kinase inhibition in diffuse large B-cell lymphoma: molecular basis for antitumor activity and drug resistance of dasatinib. Leukemia 2008 Sep; 22(9): 1755-1766.

Cheng L, Zhang DY, editors. Essentials of Molecular Genetic Pathology (2007). New York, Humana/Springer Press;.

Ye F, Jiang S, Volshonok H, Wu J, Zhang D. Molecular mechanism of anti-prostate cancer activity of Scutellaria baicalensis. Nutrition & Cancer 2007; 57(1): 100-110.

Yi J, Zhang W, Zhang DY. Molecular Zipper: A Fluorescent Probe for Real-Time Isothermal DNA amplification. Nucleic Acid Research 2006; 34: 11 e81.

Zhang D, Wu J, Ye F, Feng T, Lee I, Yin B. Amplification of circularizable probes for the detection of target nucleic acids and proteins. Clin Chim Acta 2006; 363: 61-70.